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KMID : 0438219750120030613
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Korea University Medical Journal
1975 Volume.12 No. 3 p.613 ~ p.623
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The Antagonistic Effect of Colicin, the Escherichia coli Metabolite, Against Some Groups of Pathogenic Enteric Bacteria
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Abstract
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It has been well known that the mutual relationship between bacteria is mostly antagonistic, and particular interest has been brought up. when the relationship is concerned between the non-pathogenic and pathogenic bacteria.
The study has been attempted to measure the quantitative antagonistic effect of normal intestinal flora, E. coli, against some Salmonella groups. The mode of the study was staged into three, phases; 1) the direct antagonistic effect of E. Coli, 2) the preparation of colicin by means of simple Seitz filtration, and 3) the comparison of the antagonistic, effect of colicin, one obtained by simple filtration and one by trituration method. The results of colicinogenic effect of E. coli are summarized as follows:
1. The direct, antagonistic effect of_ E. coli against groups of Salmonella was more clearly measured by means of the in_vitro.techniques. than. by the in vivo techniques. E. coli showed dramatic inhibitory effect against the groups of.Salmonella.
2. Salmonella. cholerae-suis 06 (group C,) withstood to the effect of E. coli showing a great deal of resistance.
3. The length of time in the mixed state with E. coli and. the testing strains affects. the survival. possibility of the testing strains, longer the time, more the_ inhibition. In addition, 1:1,000 dilution of the mixture showed marked survival rate of the testing strains,
4 Colicin, one of the bacterial metabolites of E. coli, also. showed.. the inhibitory effect to groups, of Salmonlla, though, less, striking than the. direct antagonistic effect.
5. Colicin obtained by `means of, triturating_ the bacterial,suspension showed higher inhibitory...ef feet. to. the testing st;ains~than. the simple, filtrate. However, its, effect was. somewhat weaker_ than the, direct antagonistic effect.
specimens, shpw.ed.; )
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6. Colicii~s.ohitained,f;om,,30,.E. coliastrains. that wieFe isolated-fro l clinical
various degree of inhibitory effect. it is assumed that the virulence of the strain affects the inhibitory effect.
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